Program Type

Undergraduate

Faculty Advisor

Dr. Suparna Chatterjee, Dr. Suresh Subedi

Document Type

Poster

Location

Face-to-face

Start Date

25-4-2023 2:45 PM

Abstract

Many native plants are used for the treatment of various diseases. Mainly those species in high chemical compound plant families can have antimicrobial properties. We selected two native plants in Arkansas, Devil’s walking stick (Aralia spinosa), and Winged sumac (Rhus copallinum), and tested them for antibacterial properties. We used three gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus epidermidis) and three gram-negative bacteria (Alcaligenes faecalis, Escherichia coli, and Serratia marcescens). The disc diffusion method is employed to identify any potential antibacterial properties for the two plant species. For this experiment, 6.50 g of dehydrated plant material (leaves of each plant species) was combined with 50 mL of 75% ethanol creating their respective tinctures which were processed to remove alcohol and make power samples. The antibacterial activity of the powders in sterile Milli-Q water was tested against 75% ethanol and hydrogen peroxide controls. After 24 and 48 hours of incubation at 37°C, the zones of inhibition were measured for each bacteria/plant sample combination. The plant samples were tested for inhibition of each bacterial species. We used nested ANOVA (analysis of variance) to examine the effect of different concentrations of plant samples and two different incubation times (24h and 48h) of each plant species on zones of inhibition for six different bacteria. Preliminary investigations showed antibacterial properties in the samples. This indicates that native plant species can have potential medicinal properties.

Included in

Microbiology Commons

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Apr 25th, 2:45 PM

Antibacterial properties of Devil’s Walking Stick and Winged Sumac extracts

Face-to-face

Many native plants are used for the treatment of various diseases. Mainly those species in high chemical compound plant families can have antimicrobial properties. We selected two native plants in Arkansas, Devil’s walking stick (Aralia spinosa), and Winged sumac (Rhus copallinum), and tested them for antibacterial properties. We used three gram-positive bacteria (Bacillus cereus, Bacillus subtilis, and Staphylococcus epidermidis) and three gram-negative bacteria (Alcaligenes faecalis, Escherichia coli, and Serratia marcescens). The disc diffusion method is employed to identify any potential antibacterial properties for the two plant species. For this experiment, 6.50 g of dehydrated plant material (leaves of each plant species) was combined with 50 mL of 75% ethanol creating their respective tinctures which were processed to remove alcohol and make power samples. The antibacterial activity of the powders in sterile Milli-Q water was tested against 75% ethanol and hydrogen peroxide controls. After 24 and 48 hours of incubation at 37°C, the zones of inhibition were measured for each bacteria/plant sample combination. The plant samples were tested for inhibition of each bacterial species. We used nested ANOVA (analysis of variance) to examine the effect of different concentrations of plant samples and two different incubation times (24h and 48h) of each plant species on zones of inhibition for six different bacteria. Preliminary investigations showed antibacterial properties in the samples. This indicates that native plant species can have potential medicinal properties.