Program Type

Undergraduate

Faculty Advisor

Dr. Suparna Chatterjee, Dr. Suresh Subedi

Document Type

Poster

Location

Face-to-face

Start Date

25-4-2023 11:30 AM

Abstract

American beautyberry (Callicarpa Americana), Sugarberry (Celtis laevigata) and Red buckeye (Aesculus pavia) are native plants of Arkansas. Plant families with species having high chemical compounds can have antimicrobial properties. In this study, the antibacterial properties of the plants are investigated against three gram positive (Bacillus cereus, Bacillus subtilis, and Staphylococcus epidermidis) and three negative bacteria (Alcaligenes faecalis, Escherichia coli, and Serratia marcescens). The disc diffusion assay method is employed to identify any potential antibacterial properties of the plants. For this experiment, 6.41 g of dehydrated plant material (leaves for both species) was combined with 50 mL of 75% ethanol creating their respective tinctures which were processed to remove alcohol and make powder samples. The antibacterial activity of the powders in sterile Milli-Q water was tested against 75% ethanol and hydrogen peroxide controls. After 24 and 48 hours of incubation at 37°C the zones of inhibition were measured for each bacteria/plant sample combination. The three species of plant samples were tested for inhibition for the six bacteria. One-way ANOVA (analysis of variance) results showed that to examine the effect of different concentrations of plant samples and two different incubation times (24h and 48h) of each plant species on zones of inhibition for six different bacteria. Sugarberry and Red buckeye (leaves) showed antibacterial effects, however, the effect was less compared with commercial antibiotics (Erythromycin and NA30). American Beautyberry has an antibacterial effect comparable to commercial antibiotics. All species leaf extracts did not differ on their antibacterial properties on gram positive and gram-negative bacteria.

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Apr 25th, 11:30 AM

Antibacterial Activity of Native Plants in Arkansas

Face-to-face

American beautyberry (Callicarpa Americana), Sugarberry (Celtis laevigata) and Red buckeye (Aesculus pavia) are native plants of Arkansas. Plant families with species having high chemical compounds can have antimicrobial properties. In this study, the antibacterial properties of the plants are investigated against three gram positive (Bacillus cereus, Bacillus subtilis, and Staphylococcus epidermidis) and three negative bacteria (Alcaligenes faecalis, Escherichia coli, and Serratia marcescens). The disc diffusion assay method is employed to identify any potential antibacterial properties of the plants. For this experiment, 6.41 g of dehydrated plant material (leaves for both species) was combined with 50 mL of 75% ethanol creating their respective tinctures which were processed to remove alcohol and make powder samples. The antibacterial activity of the powders in sterile Milli-Q water was tested against 75% ethanol and hydrogen peroxide controls. After 24 and 48 hours of incubation at 37°C the zones of inhibition were measured for each bacteria/plant sample combination. The three species of plant samples were tested for inhibition for the six bacteria. One-way ANOVA (analysis of variance) results showed that to examine the effect of different concentrations of plant samples and two different incubation times (24h and 48h) of each plant species on zones of inhibition for six different bacteria. Sugarberry and Red buckeye (leaves) showed antibacterial effects, however, the effect was less compared with commercial antibiotics (Erythromycin and NA30). American Beautyberry has an antibacterial effect comparable to commercial antibiotics. All species leaf extracts did not differ on their antibacterial properties on gram positive and gram-negative bacteria.