Unique Presentation Identifier:
P09
Program Type
Undergraduate
Faculty Advisor
Dr. Newton P. Hilliard Jr.
Document Type
Poster
Location
Face-to-face
Start Date
29-4-2025 9:30 AM
Abstract
Alkaline phosphatase (ALP) is an enzyme responsible for the dephosphorylation of extra-cytoplasmic molecules. Conversion to a neutral molecule enhances their ability to pass through cell membranes. When microbes such as Yersinia enterocolitica are ingested, they are exposed to large changes in pH level. These changes range from ~pH6.5 in the oral cavity to pH 2 in the stomach and pH 8 in the mid-intestine. The ability of microorganisms to survive this change in pH it largely dependent on their ability to change which proteins are expressed at differing pH levels or their ability to modify those proteins to meet the changing conditions. The Y. enterocolitica alkaline phosphatase (ALP) gene has been subjected to PCR and cloned into the pF1K plasmid for expression isolation and characterization. Once the enzyme is purified, it will be used as an affinity chromatography ligand to isolate the ALP modifying enzyme for further characterization.
Recommended Citation
Arellano, Flavio E., "Cloning the Yersinia enterocolitica Alkaline Phosphatase for an Affinity Chromatography Ligand" (2025). ATU Student Research Symposium. 44.
https://orc.library.atu.edu/atu_rs/2025/2025/44
Included in
Biochemistry, Biophysics, and Structural Biology Commons, Cell and Developmental Biology Commons, Immunology and Infectious Disease Commons
Cloning the Yersinia enterocolitica Alkaline Phosphatase for an Affinity Chromatography Ligand
Face-to-face
Alkaline phosphatase (ALP) is an enzyme responsible for the dephosphorylation of extra-cytoplasmic molecules. Conversion to a neutral molecule enhances their ability to pass through cell membranes. When microbes such as Yersinia enterocolitica are ingested, they are exposed to large changes in pH level. These changes range from ~pH6.5 in the oral cavity to pH 2 in the stomach and pH 8 in the mid-intestine. The ability of microorganisms to survive this change in pH it largely dependent on their ability to change which proteins are expressed at differing pH levels or their ability to modify those proteins to meet the changing conditions. The Y. enterocolitica alkaline phosphatase (ALP) gene has been subjected to PCR and cloned into the pF1K plasmid for expression isolation and characterization. Once the enzyme is purified, it will be used as an affinity chromatography ligand to isolate the ALP modifying enzyme for further characterization.