Unique Presentation Identifier:
P59
Program Type
Undergraduate
Faculty Advisor
Rajib Choudhury
Document Type
Poster
Location
Face-to-face
Start Date
29-4-2025 3:00 PM
Abstract
In this project, a simple, inexpensive colorimetric and fluorometric method for determining hydrogen peroxide in aqueous samples is described. In this method, aryl boronic acid is de-protected to produce a red dye that absorbs and emits in the red/near infrared region of the electromagnetic spectrum. Upon deprotection by the hydrogen peroxide, an intramolecular charge transfer occurs between a strong phenolate donor and a TCF acceptor to produce the strong absorption and bright fluorescence. This allows naked eye detection of hydrogen peroxide within seconds in water under physiological conditions. The detection limit is impressive and falls in the sub-micromolar (µM) range. The fluorometric detection method is unaffected by the presence of various salts, metal ions, and other interfering species commonly found in biological samples. This novel way of generating a fluorogenic donor-acceptor pair holds potential for this dye and other related derivatives for understanding the role of hydrogen peroxide in the environment, physiology, and pathology. This method's simplicity and cost-effectiveness will make it highly accessible for various applications, including environmental monitoring and medical diagnostics. Its robustness to common interferences ensures reliable results, paving the way for further research into hydrogen peroxide's impact on biological and ecological systems.
Recommended Citation
Surles, Nathan F. and Edwards, Kaitlin A., "Dual Colorimetric and Fluorometric Detection of Hydrogen Peroxide in Aqueous Samples" (2025). ATU Student Research Symposium. 48.
https://orc.library.atu.edu/atu_rs/2025/2025/48
Included in
Dual Colorimetric and Fluorometric Detection of Hydrogen Peroxide in Aqueous Samples
Face-to-face
In this project, a simple, inexpensive colorimetric and fluorometric method for determining hydrogen peroxide in aqueous samples is described. In this method, aryl boronic acid is de-protected to produce a red dye that absorbs and emits in the red/near infrared region of the electromagnetic spectrum. Upon deprotection by the hydrogen peroxide, an intramolecular charge transfer occurs between a strong phenolate donor and a TCF acceptor to produce the strong absorption and bright fluorescence. This allows naked eye detection of hydrogen peroxide within seconds in water under physiological conditions. The detection limit is impressive and falls in the sub-micromolar (µM) range. The fluorometric detection method is unaffected by the presence of various salts, metal ions, and other interfering species commonly found in biological samples. This novel way of generating a fluorogenic donor-acceptor pair holds potential for this dye and other related derivatives for understanding the role of hydrogen peroxide in the environment, physiology, and pathology. This method's simplicity and cost-effectiveness will make it highly accessible for various applications, including environmental monitoring and medical diagnostics. Its robustness to common interferences ensures reliable results, paving the way for further research into hydrogen peroxide's impact on biological and ecological systems.