Document Type
Article
Publication Date
11-1-2021
Department
Biological Sciences
Abstract
Body tissue and venom glands from an eastern population of the scorpion Centruroides vittatus (Say, 1821) were homogenized and molecular constituents removed to characterize putative sodium β toxin gene diversity, RT-qPCR, transcriptomic, and proteomic variation. We cloned sodium β toxins from genomic DNA, conducted RT-qPCR experiments with seven sodium β toxin variants, performed venom gland tissue RNA-seq, and isolated venom proteins for mass spectrophotometry. We identified greater than 70 putative novel sodium β toxin genes, 111 toxin gene transcripts, 24 different toxin proteins, and quantified sodium β toxin gene expression variation among individuals and between sexes. Our analyses contribute to the growing evidence that venom toxicity among scorpion taxa and their populations may be associated with toxin gene diversity, specific toxin transcripts variation, and subsequent protein production. Here, slight transcript variation among toxin gene variants may contribute to the major toxin protein variation in individual scorpion venom composition.
DOI
10.3390/toxins13110828
Publication Title
Toxins
Recommended Citation
Bowman, Aimee; Fitzgerald, Chloe; Pummill, Jeff F.; Rhoads, Douglas D.; and Yamashita, Tsunemi, "Reduced Toxicity of Centruroides vittatus (Say, 1821) May Result from Lowered Sodium β Toxin Gene Expression and Toxin Protein Production" (2021). Faculty Publications - Biological Sciences. 70.
https://orc.library.atu.edu/faculty_pub_biol/70
Comments
Original Citation: Bowman, A., Fitzgerald, C., Pummill, J. F., Rhoads, D. D., & Yamashita, T. (2021). Reduced Toxicity of Centruroides vittatus (Say, 1821) May Result from Lowered Sodium β Toxin Gene Expression and Toxin Protein Production. Toxins, 13(11), 828.
This article is published under a Creative Commons Attribution 4.0 International License.