Clinical Mycobacterium Abscessus Strain Inhibits Autophagy Flux and Promotes its Growth in Murine Macrophages

Document Type

Article

Publication Date

11-2017

Department

Biological & Earth Sciences

Abstract

Abstract

Autophagy is known to be a vital homeostatic defense process that controls mycobacterial infection. However, the relationship between autophagy response and the virulence of Mycobacterium abscessus strain UC22 has not been reported. Here, we demonstrate that M. abscessus induces autophagy and inhibits autophagy flux in murine macrophages. Further, the rough variant of M. abscessus, UC22 that is a highly virulent clinical isolate, significantly inhibited autophagic flux than the smooth variant of M. abscessus ATCC 19977. In addition, it was noticed that the intracellular survival of UC22 is significantly enhanced by blocking the autophagosome–lysosome fusion in macrophages compared to the smooth variant. However, Mycobacterium smegmatis did not block autophagy flux in murine macrophages. Besides, we confirmed that the lipid components of M. abscessus UC22 play a role in autophagosome formation. These data suggest that the virulent M. abscessus might be able to survive and grow within autophagosomes by preventing the autophagosome–lysosome fusion and their clearance from the cells.

DOI

https://doi.org/10.1093/femspd/ftx107

First Page

ftx 107

Publication Title

Pathogens and Disease

Comments

At the time of publication, Dr. Bindu Subhadra was affiliated with Chungnam National University.

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