Document Type

Article

Publication Date

2013

Department

Biological & Earth Sciences

Abstract

The cyclic AMP receptor protein (CRP) homolog, GlxR, controls the expression of several genes involved in the regulation of diverse physiological processes in Corynebacterium glutamicum. In silico analysis has revealed the presence of glxR binding sites upstream of genes ptsG, adhA, and ald, encoding glucose-specific phosphotransferase system protein, alcohol dehydrogenase (ADH), and acetaldehyde dehydrogenase (ALDH), respectively. However, the involvement of the GlxR-cAMP complex on the expression of these genes has been explored only in vitro. In this study, the expressions of ptsG, adhA, and ald were analyzed in detail using an adenylate cyclase gene (cyaB) deletion mutant and glxR deletion mutant. The specific activities of ADH and ALDH were increased in both the mutants in glucose and glucose plus ethanol media, in contrast to the wild type. In accordance, the promoter activities of adhA and ald were derepressed in the cyaB mutant, indicating that glxR acts as a repressor of adhA. Similarly, both the mutants exhibited derepression of ptsG regardless of the carbon source. These results confirm the involvement of GlxR on the expression of important carbon metabolic genes; adhA, ald, and ptsG.

DOI

https://doi.org/10.4014/jmb.1310.10031

First Page

1683

Last Page

1690

Publication Title

Journal of Microbiology and Biotechnology

Publisher

Journal of Microbiology and Biotechnology

Comments

Original Citation:

Subhadra, B., Lee, J.K. Elucidation of the regulation of ethanol catabolic genes and ptsG using a glxR and adenylate cyclase gene (cyaB) deletion mutants of Corynebacterium glutamicum ATCC 13032. Journal of Microbiology and Biotechnology. 2013;23:1683-1690. https://doi.org/10.4014/jmb.1310.10031

This is an open-access article.

At the time of publication, Dr. Bindu Subhadra was affiliated with Paichai University.

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