Bioactive Withanolides from Withania obtusifolia
Document Type
Article
Publication Date
1-1-2014
Department
Physical & Earth Sciences
Abstract
Seven withanolides were isolated from the leaves of Withania obtusifolia. Of these, one was new [obtusifonolide (1)], five were new to the species [sitoindoside IX (2), 6α-chloro-5β-hydroxy withaferin A (3), isowithanone (4), 2,3-dihydro-3-ethoxywithaferin A (5), and daturataturin A (6)], and one was reported previously from W. obtusifolia [withaferin A (7)]. The structures were elucidated using a set of spectroscopic and spectrometric techniques. Compounds (1-7) were evaluated for cytotoxicity against a human cancer cell panel and for antimicrobial activity in an array of bacteria and fungi. Compound 7 showed cytotoxic activity against the MDA-MB-435 (human melanoma) and SW-620 (human colon cancer) cell lines with IC50 values of 1.7 and 0.3 μM, respectively. The in vitro activity of 7 on 17β-hydroxysteroid dehydrogenase and 5α-reductase was also investigated. © 2014 Phytochemical Society of Europe.
DOI
10.1016/j.phytol.2014.05.002
First Page
96
Last Page
101
Volume
9
Issue
1
Publication Title
Phytochemistry Letters
ISSN
18743900
Recommended Citation
Alali, F. Q., Amrine, C. S. M., El-Elimat, T., Alkofahi, A., Tawaha, K., Gharaibah, M., Swanson, S. M., Falkinham, J. O., Cabeza, M., Sánchez, A., Figueroa, M., & Oberlies, N, H. (2014). Bioactive withanolides from Withania obtusifolia. Phytochemistry Letters, 9(1): 96-101. doi: 10.1016/j.phytol.2014.05.002
Comments
At the time of publication, Chiraz Soumia M. Amrine was affiliated with Jordan University of Science and Technology.