Document Type
Article
Publication Date
4-2019
Department
Biological & Earth Sciences
Abstract
NemR is an electrophile-sensing regulator which controls two enzymes required for the detoxification of reactive electrophiles: N-ethylmaleimide (NEM) reductase and glyoxalase I in Escherichia coli. Both enzymes are essential for bacterial survival in the presence of toxic reactive electrophiles, such as N-ethylmaleimide and methyl glyoxal. Here, we report the identification and characterization of NemR from Acinetobacter nosocomialis, a nosocomial pathogen. We confirmed that nemR and the nemaA gene which encodes N-ethylmaleimide reductase form a single operon, which is in accordance with the reports from E. coli. Bioinformatic analysis revealed the presence of an NemR binding motif in the promoter regions of nemRA operon and gloA (encoding glyoxalase I) and the binding was confirmed by gel mobility shift assay. The deletion of nemR resulted in increased biofilm/pellicle formation in A. nosocomialis. mRNA expression analysis revealed that NemR acts as a repressor of the nemRA operon and gloA, and that the repressor function is inactivated by the addition of toxic Cys modification agents, contributing to bacterial survival. In addition, it was demonstrated that the nemRA operon is positively regulated by the quorum sensing regulator, AnoR and the operon plays a role in biofilm/pellicle formation in A. nosocomialis.
DOI
https://doi.org/10.1016/j.resmic.2019.02.001
First Page
123
Last Page
130
Publication Title
Research in Microbiology
Recommended Citation
Subhadra, Bindu; Surendran, Surya; Kim, Dong Ho; Woo, Kyungho; Oh, Man Hwan; and Choi, Chul Hee, "The transcription factor NemR is an electrophile-sensing regulator important for the detoxification of reactive electrophiles in Acinetobacter nosocomialis" (2019). Faculty Publications - Biological Sciences. 106.
https://orc.library.atu.edu/faculty_pub_biol/106
Comments
Original Citation:
Subhadra, B., Surendran S., Kim D.H., Woo K., Oh M.H., Choi C.H. (2019). The transcription factor NemR is an electrophile-sensing regulator important for the detoxification of reactive electrophiles in Acinetobacter nosocomialis. Research in Microbiology, 170(3):123-30.
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
At the time of publication, Dr. Bindu Subhadra was affiliated with Chungnam National University.